Juan Sebastian Yakisich

 

 

Laboratory Management

 

 

Budgets (Research grants)

Program and Project Management - Grantsmanship

Research Design (planning, execution & publication)

Research Communication (articles, reviews, book chapters, books)

Team Building​ (Teaching & research groups)

Training and mentoring staff and students (Undergraduate, graduate, Master & PhD)

 
 

Knowledge Areas

 
 

Cell & Molecular Biology (with focus on tumor biology)

Biochemistry

Oncology

Pharmacology

Preclinical Anticancer Drug Screening

Cancer Stem Cells

 
 

Technical Skills

 

Cell Biology

 

 

 

Flow cytometry / Cell cycle analysis  / Cell and tissue culture / Cell death analysis (apoptosis, autophagy) / Short term proliferation assay (e.g. CCK) / Long-term proliferation assay / Senescence assay (β-galactosidase) / Cell migration assay

 

 

Molecular Biology

 

 

 

 

DNA (plasmid and genomic) and RNA purification / Cloning and site-directed mutagenesis / PCR, RT-PCR, single cell PCR, qPCR arrays / Northern and Southern blot  / Chromatin immunoprecipitation / Pulse field electrophoresis / Biolistic transformation

 

 

Proteins

 

 

 

Purification, immunoprecipitation

Western Blot

2D-PAGE

 

 

Lipids

 

Extraction/ purification / TLC

 

 

Biochemistry

 

Enzymatic activity (topoisomerase I and II activity, protein kinase activity)

 

 

Microscopy

 

Fluorescence, Light / Histo and cytochemistry /Confocal Microscopy

 

 

Animal models

 

Cancer (RG2 rat glioma model) / Diabetes (Streptozotocin)

 

 

Miscellaneous

 

Radiolabelling (protein, lipids, DNA, posttranslational modifications: phosphorylation, glycosylation)

 

     

 

   

JSY

 

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JSY